A Wound Healing Formulation Based on Iranian Traditional Medicine and Its HPTLC Fingerprint

The present study reports formulation of a traditionally used poly herbal product for wound healing and its chromatographic analysis by HPTLC. Herbal therapy was the common treatment prescribed by Iranian physicians for wound healing. “Zemad”, the most ancient pharmaceutical dosage form used in the Iranian Traditional Medicine (ITM) for skin diseases. In this study, a poly herbal paste (PHP) with hydrophilic base, according to the ITM has been made for wound healing. Powder of Aloe vera gel, oleo gum resins of Boswellia carteri and Commiphora myrrha were used in PHP. PHP were made base on Hydrogel. The physical stability of the formulation was evaluated by testing the physical changes like phase separation, color, odor, and consistency. The HPTLC fingerprint has been used efficiently for the identification and quality assessment of the formulation, also proving the presence of the mentioned herbs in the formula. In order to extract PHP components for HPTLC, PHP was extracted with methanol. Methanol fraction of A. vera, C. myrrha and B. carterri were used as standard materials. Stability and rheological behavior evaluation as well as microbiological tests showed that the prepared formulation was stable with no growth of pathogenic microorganisms and suitable for topical application. HPTLC fingerprinting of PHP confirmed the presence of compounds corresponding to each of the three plants used in the formula. Regarding to, the antioxidant, anti-inflammatory and antimicrobial effects of the constituents of PHP, the product could be an appropriate candidate for wound healing with respect to its traditional use in ITM.